Muscle Actin Antibody [HHF35]

• Background:

  This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas) .

• NCBI Gene ID:

  58

• Swiss Prot:

  P68133

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rabbit, Rat

• Immunogen:

  SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.

• Clonality:

  Monoclonal

• Clone:

  HHF35

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  ACTA1

• NCBI Official Name:

  Actin, alpha 1, skeletal muscle

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA

• Tested Applications:

  WB, Flow, IHC, IF

• Physical Properties:

  Liquid