P53 Antibody [DO-7]

• Background :

  This antibody is specific for a 53kDa protein, which is identified as p53 suppressor gene product. The large number of prolines in its amino acid sequence causes p53 to migrate slowly in SDS-PAGE, resulting in the amino acid content-estimated 43 kDa protein appearing larger than expected. DO-7 reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intra-tubular germ cell neoplasia.

• NCBI Gene ID :

  7157

• Swiss Prot :

  P04637

• Host :

  Mouse

• Reactivity :

  Human, Monkey, Bovine

• Immunogen :

  Recombinant human wild type p53 expressed in E. coli was used as the immunogen for this antibody. Its epitope maps within the N-terminus portion (aa 20-25) of the p53 oncoprotein.

• Clonality :

  Monoclonal

• Clone :

  DO-7

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle, Signal Transduction

• Purification :

  Protein G affinity chromatography

• Concentration :

  0.2 mg/mL

• Buffer :

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment :

  IgG2b, kappa

• Applications Notes :

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol :

  TP53

• NCBI Official Name :

  Tumor protein p53

• NCBI Organism :

  Homo sapiens

• Other Product Names :

  Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

• Tested Applications :

  WB, Flow, IHC, IF

• Physical Properties :

  Liquid