P27Kip1 Antibody [SX53G8]

• Background:

  This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

• NCBI Gene ID:

  1027

• Swiss Prot:

  P46527

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Monkey

• Immunogen:

  Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.

• Clonality:

  Monoclonal

• Clone:

  SX53G8

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  CDKN1B

• NCBI Official Name:

  Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1

• Tested Applications:

  WB, Flow, IHC, IF

• Physical Properties:

  Liquid