P27Kip1 (phospho Thr187) Antibody

• Background:

  Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

• NCBI Gene ID:

  1027

• Swiss Prot:

  P46527

• Accession Number:

  NP_004055.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  P27Kip1 (Phospho-Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  27 kDa

• Specificity:

  This antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  CDKN1B

• NCBI Official Name:

  Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Eguchi H, et al. (2003) Cancer Res; 63 (15) : 4739-46Le XF, et al. (2003) J Biol Chem; Connor MK, et al. (2003) ) Mol Biol Cell; 14 (1) : 201-13Fujita N, et al. (2002) J Biol Chem; 277 (32) : 28706-13Ishida N, et al. (2000) J Biol Chem; 275 (33) : 25146-54

• Other Product Names:

  KIP1, MEN4, CDKN4, MEN1B, P27KIP1, MAPTL, MTBT1, TAU, CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1

• Tested Applications:

  WB, IHC

• Protein ID:

  4757962

• Physical Properties:

  Liquid