UBA3 Antibody

• Background :

  UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

• NCBI Gene ID :

  9039

• Swiss Prot :

  Q8TBC4

• Accession Number :

  NP_003959

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBA3.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle, Cancer, Apoptosis

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1309 - Human Placenta Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UBA3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  52 kDa

• Applications Notes :

  UBA3 antibody can be used for detection of UBA3 by ELISA at 1:1562500. UBA3 antibody can be used for detection of UBA3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  UBA3

• NCBI Official Name :

  Ubiquitin-like modifier activating enzyme 3

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Li, T., (2006) Arch. Biochem. Biophys. 453 (1), 70-74 .

• Other Product Names :

  UBA3, DKFZp566J164, MGC22384, UBE1C, hUBA3, NAE2

• Tested Applications :

  ELISA, WB

• Protein ID :

  38045942

• Physical Properties :

  Liquid