NUCB1 Antibody

• Background:

  An extracellular calcium binding protein of the mineralized matrix of bone [Calnuc or RGD:620030]. Also useful as a Golgi marker in immunohistochemistry (1:100 dilution) .

• NCBI Gene ID:

  84595

• Swiss Prot:

  Q63083

• Accession Number:

  NP_445915

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic KLH-conjugated peptide corresponding a region of rat NUCB1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Antibody is purified by Protein A/G affinity chromatography method.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NUCB1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  54 kDa

• Applications Notes:

  NUCB1 antibody can be used for detection of NUCB1 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NUCB1

• NCBI Official Name:

  Nucleobindin 1

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Wendel, M., et al., 1995, J. Biol. Chem. 270 (11), 6125-6133.

• Other Product Names:

  NUCB1, Nucb

• Tested Applications:

  ELISA, WB, IHC, IP

• Protein ID:

  16758210

• Physical Properties:

  Liquid