Nuclear Antigen Antibody [NUCM1-1]

• Background:

  This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.

• Swiss Prot:

  NotKnown

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.

• Clonality:

  Monoclonal

• Clone:

  NUCM1-1

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Tested Applications:

  IF, ICC, IHC-P

• Physical Properties:

  Liquid