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AKT1 Antibody

CAT: 0223-31-052Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-31-052Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
NCBI Gene ID
207
Swiss Prot
P31749
Accession Number
NP_005154
Host
Rabbit
Reactivity
Human, Mouse, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Signal Transduction, Apoptosis
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AKT1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
56 kDa
Applications Notes
AKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
AKT1
NCBI Official Name
V-akt murine thymoma viral oncogene homolog 1
NCBI Organism
Homo sapiens
Background Reference 01
Kim, H.A., et al., (2006) Exp. Mol. Pathol. 80 (2), 165-170.
Other Product Names
AKT1, AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Tested Applications
ELISA, WB
Protein ID
62241011
Physical Properties
Liquid

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