AKT1 Antibody

• Background:

  AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

• NCBI Gene ID:

  207

• Swiss Prot:

  P31749

• Accession Number:

  NP_005154

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction, Apoptosis

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AKT1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  56 kDa

• Applications Notes:

  AKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  AKT1

• NCBI Official Name:

  V-akt murine thymoma viral oncogene homolog 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kim, H.A., et al., (2006) Exp. Mol. Pathol. 80 (2), 165-170.

• Other Product Names:

  AKT1, AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA

• Tested Applications:

  ELISA, WB

• Protein ID:

  62241011

• Physical Properties:

  Liquid