CDKN2B Antibody

• Background:

  CDKN2B's gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. CDKN2B is a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition.

• NCBI Gene ID:

  1030

• Swiss Prot:

  P42772

• Accession Number:

  NP_004927

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDKN2B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer, Apoptosis

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CDKN2B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  15 kDa

• Applications Notes:

  CDKN2B antibody can be used for detection of CDKN2B by ELISA at 1:2500. CDKN2B antibody can be used for detection of CDKN2B by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CDKN2B

• NCBI Official Name:

  Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Scott, S.A., et al., (2004) Leuk. Res. 28 (12), 1293-1301.

• Other Product Names:

  CDKN2B, P15, MTS2, TP15, CDK4I, INK4B, p15INK4b

• Tested Applications:

  ELISA, WB

• Protein ID:

  17981694

• Physical Properties:

  Liquid