P4HB Antibody

P4HB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

5034

P07237

NP_000909

Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human P4HB.

Unconjugated

Primary Antibodies

Signal Transduction

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1211 - HepG2 Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store P4HB antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

55 kDa

P4HB antibody can be used for detection of P4HB by ELISA at 1:62500. P4HB antibody can be used for detection of P4HB by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

P4HB

Prolyl 4-hydroxylase, beta polypeptide

Homo sapiens

Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007) .

P4HB, DSI, ERBA2L, GIT, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, P4Hbeta

ELISA, WB, IHC

20070125