PDHA1 Antibody

• Background:

  The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1) ; dihydrolipoyl transacetylase (DLAT) ; and dihydrolipoyl dehydrogenase (DLD) . The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1) ; dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12) ; and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4) . The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]) .[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

• NCBI Gene ID:

  5160

• Swiss Prot:

  P08559

• Accession Number:

  NP_000275

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDHA1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDHA1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  40 kDa

• Applications Notes:

  PDHA1 antibody can be used for detection of PDHA1 by ELISA at 1:312500. PDHA1 antibody can be used for detection of PDHA1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PDHA1

• NCBI Official Name:

  Pyruvate dehydrogenase (lipoamide) alpha 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Boichard, A., (2008) Mol. Genet. Metab. 93 (3), 323-330.

• Other Product Names:

  PDHA1, PDHA, PDHCE1A, PHE1A

• Tested Applications:

  ELISA, WB

• Protein ID:

  4505685

• Physical Properties:

  Liquid