PDHA1 Antibody

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1) ; dihydrolipoyl transacetylase (DLAT) ; and dihydrolipoyl dehydrogenase (DLD) . The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1) ; dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12) ; and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4) . The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]) .[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

5160

P08559

NP_000275

Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDHA1.

Unconjugated

Primary Antibodies

Other

Antibody is purified by protein A chromatography method.

Cat. No. 1211 - HepG2 Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDHA1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

40 kDa

PDHA1 antibody can be used for detection of PDHA1 by ELISA at 1:312500. PDHA1 antibody can be used for detection of PDHA1 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

PDHA1

Pyruvate dehydrogenase (lipoamide) alpha 1

Homo sapiens

Boichard, A., (2008) Mol. Genet. Metab. 93 (3), 323-330.

PDHA1, PDHA, PDHCE1A, PHE1A

ELISA, WB

4505685