UBE2D2 Antibody

• Background:

  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.SLC38A4 is found predominantly in liver and transports both cationic and neutral amino acids. The transport of cationic amino acids by SLC38A4 is Na (+) and pH independent, while the transport of neutral amino acids is Na (+) and pH dependent (Hatanaka et al., 2001) .

• NCBI Gene ID:

  7322

• Swiss Prot:

  P62837

• Accession Number:

  NP_003330

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2D2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Apoptosis, Homeostasis

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UBE2D2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  60 kDa, 61 kDa

• Applications Notes:

  UBE2D2 antibody can be used for detection of UBE2D2 by ELISA at 1:1562500. UBE2D2 antibody can be used for detection of UBE2D2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  UBE2D2

• NCBI Official Name:

  Ubiquitin-conjugating enzyme E2D 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Desforges, M., Am. J. Physiol., Cell Physiol. 290 (1), C305-C312 (2006) .

• Other Product Names:

  UBE2D2, E2 (17) KB2, PUBC1, UBC4, UBC4/5, UBCH5B

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4507775

• Physical Properties:

  Liquid