PRPF19 Antibody

• Background:

  PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for spliceosome binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. It also may have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.

• NCBI Gene ID:

  27339

• Swiss Prot:

  Q9UMS4

• Accession Number:

  NP_055317

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRPF19.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Homeostasis

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRPF19 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  55 kDa

• Applications Notes:

  PRPF19 antibody can be used for detection of PRPF19 by ELISA at 1:1562500. PRPF19 antibody can be used for detection of PRPF19 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PRPF19

• NCBI Official Name:

  Pre-mRNA processing factor 19

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Voglauer, R., (2006) Exp. Cell Res. 312 (6), 746-759.

• Other Product Names:

  PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  7657381

• Physical Properties:

  Liquid