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PBEF1 Antibody

CAT: 0223-29-725Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-29-725Size:100 µL
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Background
PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
NCBI Gene ID
10135
Swiss Prot
P43490
Accession Number
NP_005737
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PBEF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PBEF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
54 kDa
Applications Notes
PBEF1 antibody can be used for detection of PBEF1 by ELISA at 1:312500. PBEF1 antibody can be used for detection of PBEF1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
NAMPT
NCBI Official Name
Nicotinamide phosphoribosyltransferase
NCBI Organism
Homo sapiens
Background Reference 01
Chen, M.P., (2006) J. Clin. Endocrinol. Metab. 91 (1), 295-299.
Other Product Names
PBEF1, 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN
Tested Applications
ELISA, WB
Protein ID
5031977
Physical Properties
Liquid

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