CYP2D6 Antibody

• Background:

  CYP2D6 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 20% of commonly prescribed drugs. Its substrates include debrisoquine, an adrenergic-blocking drug; sparteine and propafenone, both anti-arrythmic drugs; and amitryptiline, an anti-depressant.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 20% of commonly prescribed drugs. Its substrates include debrisoquine, an adrenergic-blocking drug; sparteine and propafenone, both anti-arrythmic drugs; and amitryptiline, an anti-depressant. The gene is highly polymorphic in the population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  1565

• Swiss Prot:

  P10635

• Accession Number:

  NP_000097

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYP2D6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Cancer, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. XBL-10409 - Fetal Liver Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CYP2D6 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  55 kDa

• Applications Notes:

  CYP2D6 antibody can be used for detection of CYP2D6 by ELISA at 1:312500. CYP2D6 antibody can be used for detection of CYP2D6 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CYP2D6

• NCBI Official Name:

  Cytochrome P450, family 2, subfamily D, polypeptide 6

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rowland, P., (2006) J. Biol. Chem. 281 (11), 7614-7622.

• Other Product Names:

  CYP2D6, RP4-669P10.2, CPD6, CYP2D, CYP2D@, CYP2DL1, MGC120389, MGC120390, P450-DB1, P450C2D, CYPIID6, P450DB1, CYP2D7AP, CYP2D7BP, CYP2D7P2, CYP2D8P2

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  392513721

• Physical Properties:

  Liquid