SF1 Antibody

• Background:

  SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.

• NCBI Gene ID:

  7536

• Swiss Prot:

  Q15637

• Accession Number:

  NP_004621

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  68 kDa, 69 kDa, 9 kDa, 60 kDa

• Applications Notes:

  SF1 antibody can be used for detection of SF1 by ELISA at 1:312500. SF1 antibody can be used for detection of SF1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SF1

• NCBI Official Name:

  Splicing factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Huang, Y.H., (2006) Endocrinology 147 (5), 2481-2489.

• Other Product Names:

  SF1, BBP, MBBP, ZFM1, ZNF162, D11S636, ZCCHC25

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  42544130

• Physical Properties:

  Liquid