SF3B4 Antibody

SF3B4 is one of four subunits of the splicing factor 3B. The protein cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

10262

Q15427

NP_005841

Human, Mouse, Rat, Dog, Zebrafish

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B4.

Unconjugated

Primary Antibodies

Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1205 - Jurkat Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF3B4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

47 kDa

SF3B4 antibody can be used for detection of SF3B4 by ELISA at 1:312500. SF3B4 antibody can be used for detection of SF3B4 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

SF3B4

Splicing factor 3b, subunit 4, 49kDa

Homo sapiens

Nishanian, T.G. (2004) Biochem. Biophys. Res. Commun. 323 (1), 91-97.

SF3B4, AFD1, Hsh49, SAP49, SF3b49

ELISA, WB

5032069