SF3B1 Antibody

SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

23451

O75533

NP_036565

Human, Mouse, Rat, Dog

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B1.

Unconjugated

Primary Antibodies

Other

Antibody is purified by protein A chromatography method.

Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF3B1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

143 kDa, 16 kDa

SF3B1 antibody can be used for detection of SF3B1 by ELISA at 1:62500. SF3B1 antibody can be used for detection of SF3B1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

SF3B1

Splicing factor 3b, subunit 1, 155kDa

Homo sapiens

Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.

SF3B1, PRP10, SAP155, SF3b155, MDS, Hsh155, PRPF10

ELISA, WB, IHC

54112117