NCL Antibody

• Background:

  Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

• NCBI Gene ID:

  4691

• Swiss Prot:

  P19338

• Accession Number:

  NP_005372

• Host:

  Rabbit

• Reactivity:

  Human, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCL.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NCL antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  78 kDa

• Applications Notes:

  NCL antibody can be used for detection of NCL by ELISA at 1:312500. NCL antibody can be used for detection of NCL by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NCL

• NCBI Official Name:

  Nucleolin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Huang, Y., (2006) Blood 107 (9), 3564-3571.

• Other Product Names:

  NCL, C23

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  55956788

• Physical Properties:

  Liquid