U2AF2 Antibody

• Background :

  U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

• NCBI Gene ID :

  11338

• Swiss Prot :

  Q96HC5

• Accession Number :

  NP_001012496

• Host :

  Rabbit

• Reactivity :

  Human, Rat

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human U2AF2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store U2AF2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  52 kDa, 54 kDa

• Applications Notes :

  U2AF2 antibody can be used for detection of U2AF2 by ELISA at 1:62500. U2AF2 antibody can be used for detection of U2AF2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  U2AF2

• NCBI Official Name :

  U2 small nuclear RNA auxiliary factor 2

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Sickmier, E.A., Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62 (PT.

• Other Product Names :

  U2AF2, U2AF65

• Tested Applications :

  ELISA, WB

• Protein ID :

  60279268

• Physical Properties :

  Liquid