PRMT2 Antibody

• Background:

  The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.

• NCBI Gene ID:

  3275

• Swiss Prot:

  P55345

• Accession Number:

  NP_001526

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRMT2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  48 kDa, 49 kDa

• Applications Notes:

  PRMT2 antibody can be used for detection of PRMT2 by ELISA at 1:1562500. PRMT2 antibody can be used for detection of PRMT2 by western blot at 0.625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PRMT2

• NCBI Official Name:

  Protein arginine methyltransferase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Qi, C., (2002) J. Biol. Chem. 277 (32), 28624-28630.

• Other Product Names:

  PRMT2, HRMT1L1

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  46255047

• Physical Properties:

  Liquid