PRMT5 Antibody

• Background:

  Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

• NCBI Gene ID:

  10419

• Swiss Prot:

  O14744

• Accession Number:

  O14744

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-499 amino acids from the C-terminal region of human PRMT5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  73 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PRMT5

• NCBI Official Name:

  Protein arginine N-methyltransferase 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pal, S., et al., Mol. Cell. Biol. 23 (21) :7475-7487 (2003) .

• Background Reference 02:

  Rho, J., et al., J. Biol. Chem. 276 (14) :11393-11401 (2001) .

• Background Reference 03:

  Pollack, B.P., et al., J. Biol. Chem. 274 (44) :31531-31542 (1999) .

• Background Reference 04:

  Gilbreth, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95 (25) :14781-14786 (1998) .

• Other Product Names:

  Protein arginine N-methyltransferase 5, 211-, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally processed, PRMT5, HRMT1L5, IBP72, JBP1, SKB1

• Tested Applications:

  WB

• Physical Properties:

  Liquid