PRMT5 Antibody

• Background:

  PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs) . It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.

• NCBI Gene ID:

  10419

• Swiss Prot:

  O14744

• Accession Number:

  NP_001034708

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Homeostasis

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRMT5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  68 kDa, 73 kDa

• Applications Notes:

  PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PRMT5

• NCBI Official Name:

  Protein arginine methyltransferase 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Azzouz, T.N., (2005) J. Biol. Chem. 280 (41), 34435-34440.

• Other Product Names:

  PRMT5, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  88900507

• Physical Properties:

  Liquid