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BRD4 Antibody

CAT: 0223-29-005Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-29-005Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t (15;19) (q13; p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
NCBI Gene ID
23476
Swiss Prot
O60885-2
Accession Number
NP_055114
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store BRD4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
80 kDa, 152 kDa
Applications Notes
BRD4 antibody can be used for detection of BRD4 by ELISA at 1:1562500. BRD4 antibody can be used for detection of BRD4 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
BRD4
NCBI Official Name
Bromodomain containing 4
NCBI Organism
Homo sapiens
Background Reference 01
Schweiger, M.R., (2006) J. Virol. 80 (9), 4276-4285.
Other Product Names
BRD4, CAP, HUNKI, MCAP, HUNK1
Tested Applications
ELISA, WB
Protein ID
7657218
Physical Properties
Liquid

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