CRSP8 Antibody

He activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP8 is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

9442

Q6P2C8

NP_004260

Human, Mouse, Rat, Dog

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRSP8.

Unconjugated

Primary Antibodies

Transcription

Antibody is purified by protein A chromatography method.

Cat. No. 1211 - HepG2 Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CRSP8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

35 kDa

CRSP8 antibody can be used for detection of CRSP8 by ELISA at 1:62500. CRSP8 antibody can be used for detection of CRSP8 by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

MED27

Mediator complex subunit 27

Homo sapiens

Sato, S., et al., (2003) J. Biol. Chem. 278 (17), 15123-15127.

CRSP8, CRSP8, CRAP34, CRSP34, TRAP37

ELISA, WB

28558979