TWIST1 Antibody

• Background:

  Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation.TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

• NCBI Gene ID:

  7291

• Swiss Prot:

  Q15672

• Accession Number:

  NP_000465

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TWIST1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TWIST1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  21 kDa

• Applications Notes:

  TWIST1 antibody can be used for detection of TWIST1 by ELISA at 1:1562500. TWIST1 antibody can be used for detection of TWIST1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TWIST1

• NCBI Official Name:

  Twist family bHLH transcription factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Reinhold, M.I., et al., (2006) J. Biol. Chem. 281 (3), 1381-1388.

• Other Product Names:

  TWIST1, CRS, SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38

• Tested Applications:

  ELISA, WB

• Protein ID:

  4507741

• Physical Properties:

  Liquid