MITF Antibody

• Background:

  MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

• NCBI Gene ID:

  4286

• Swiss Prot:

  O75030-9

• Accession Number:

  NP_000239

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MITF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MITF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  47 kDa, 58 kDa, 46 kDa, 58 kDa, 56 kDa, 55 kDa

• Applications Notes:

  MITF antibody can be used for detection of MITF by ELISA at 1:1562500. MITF antibody can be used for detection of MITF by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MITF

• NCBI Official Name:

  Microphthalmia-associated transcription factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Park, H.Y., et al., (2006) Biochem. J. 395 (3), 571-578.

• Other Product Names:

  MITF, MI, WS2, CMM8, WS2A, bHLHe32

• Tested Applications:

  ELISA, WB

• Protein ID:

  4557755

• Physical Properties:

  Liquid