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MITF Antibody

CAT: 0223-28-763Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-28-763Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
NCBI Gene ID
4286
Swiss Prot
O75030-9
Accession Number
NP_000239
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MITF.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MITF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
47 kDa, 58 kDa, 46 kDa, 58 kDa, 56 kDa, 55 kDa
Applications Notes
MITF antibody can be used for detection of MITF by ELISA at 1:1562500. MITF antibody can be used for detection of MITF by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
MITF
NCBI Official Name
Microphthalmia-associated transcription factor
NCBI Organism
Homo sapiens
Background Reference 01
Park, H.Y., et al., (2006) Biochem. J. 395 (3), 571-578.
Other Product Names
MITF, MI, WS2, CMM8, WS2A, bHLHe32
Tested Applications
ELISA, WB
Protein ID
4557755
Physical Properties
Liquid

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