MITF Antibody

• Background :

  MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

• NCBI Gene ID :

  4286

• Swiss Prot :

  O75030-9

• Accession Number :

  NP_000239

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MITF.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription, Cancer

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MITF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  47 kDa, 58 kDa, 46 kDa, 58 kDa, 56 kDa, 55 kDa

• Applications Notes :

  MITF antibody can be used for detection of MITF by ELISA at 1:1562500. MITF antibody can be used for detection of MITF by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  MITF

• NCBI Official Name :

  Microphthalmia-associated transcription factor

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Park, H.Y., et al., (2006) Biochem. J. 395 (3), 571-578.

• Other Product Names :

  MITF, MI, WS2, CMM8, WS2A, bHLHe32

• Tested Applications :

  ELISA, WB

• Protein ID :

  4557755

• Physical Properties :

  Liquid