PTRF Antibody

• Background :

  In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.

• NCBI Gene ID :

  19285

• Swiss Prot :

  O54724

• Accession Number :

  NP_033012

• Host :

  Rabbit

• Reactivity :

  Mouse

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PTRF.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PTRF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  43 kDa

• Applications Notes :

  PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  PTRF

• NCBI Official Name :

  Polymerase I and transcript release factor

• NCBI Organism :

  Mus musculus

• Background Reference 01 :

  Miyoshi, K., et al., (2001) Genomics 71 (2), 150-155.

• Other Product Names :

  PTRF, Cavin, Cav-p60, AW546441, 2310075E07Rik

• Tested Applications :

  ELISA, WB

• Protein ID :

  6679567

• Physical Properties :

  Liquid