PTRF Antibody

• Background:

  In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.

• NCBI Gene ID:

  19285

• Swiss Prot:

  O54724

• Accession Number:

  NP_033012

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PTRF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PTRF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  43 kDa

• Applications Notes:

  PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PTRF

• NCBI Official Name:

  Polymerase I and transcript release factor

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Miyoshi, K., et al., (2001) Genomics 71 (2), 150-155.

• Other Product Names:

  PTRF, Cavin, Cav-p60, AW546441, 2310075E07Rik

• Tested Applications:

  ELISA, WB

• Protein ID:

  6679567

• Physical Properties:

  Liquid