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SMARCA3 Antibody

CAT: 0223-28-113Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-28-113Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
SMARCA3 (HLTF) is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA3 contains a RING finger DNA binding motif. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform which is truncated at the N-terminus as compared to the full-length protein.
NCBI Gene ID
6596
Swiss Prot
Q14527
Accession Number
NP_620636
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCA3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Tranfected 293T Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SMARCA3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
114 kDa, 114 kDa
Applications Notes
SMARCA3 antibody can be used for detection of SMARCA3 by ELISA at 1:312500. SMARCA3 antibody can be used for detection of SMARCA3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
HLTF
NCBI Official Name
Helicase-like transcription factor
NCBI Organism
Homo sapiens
Background Reference 01
Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.
Other Product Names
SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SMARCA3
Tested Applications
ELISA, WB
Protein ID
21071054
Physical Properties
Liquid

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