SMARCA3 Antibody

• Background :

  SMARCA3 (HLTF) is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA3 contains a RING finger DNA binding motif. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform which is truncated at the N-terminus as compared to the full-length protein.

• NCBI Gene ID :

  6596

• Swiss Prot :

  Q14527

• Accession Number :

  NP_620636

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCA3.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Tranfected 293T Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SMARCA3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  114 kDa, 114 kDa

• Applications Notes :

  SMARCA3 antibody can be used for detection of SMARCA3 by ELISA at 1:312500. SMARCA3 antibody can be used for detection of SMARCA3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  HLTF

• NCBI Official Name :

  Helicase-like transcription factor

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.

• Other Product Names :

  SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SMARCA3

• Tested Applications :

  ELISA, WB

• Protein ID :

  21071054

• Physical Properties :

  Liquid