SMARCA2 Antibody

• Background:

  SMARCA2 is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

• NCBI Gene ID:

  6595

• Swiss Prot:

  Q5TB75

• Accession Number:

  Q5TB75

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCA2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SMARCA2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  32 kDa

• Applications Notes:

  SMARCA2 antibody can be used for detection of SMARCA2 by ELISA at 1:312500. SMARCA2 antibody can be used for detection of SMARCA2 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SMARCA2

• NCBI Official Name:

  SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Muchardt, C., et al., (1994) Mamm. Genome 5 (4), 241-242.

• Other Product Names:

  SMARCA2, BRM, SNF2, SWI2, hBRM, NCBRS, Sth1p, BAF190, SNF2L2, SNF2LA, hSNF2a

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  74745911

• Physical Properties:

  Liquid