SMARCA4 Antibody

• Background:

  The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1) . Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2) . The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3) . In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5) .

• NCBI Gene ID:

  6597

• Swiss Prot:

  P51532

• Accession Number:

  NP_001122321

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  SMARCA4 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1220 - SK-N-SH Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SMARCA4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.

• Symbol:

  SMARCA4

• NCBI Official Name:

  SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Muchardt C and Yaniv M. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor. EMBO J. 1993; 12:4279-90.

• Background Reference 02:

  Wilson BG and Roberts CW. SWI/SNF nucleosome remodellers and cancer. Nat. Rev. Cancer 2011; 11:481-92.

• Background Reference 03:

  Bultman S, Gebuhr T, Yee D, et al. A Brg1 null mutation in the mouse reveals functional differences among mammalian SWI/SNF complexes. Mol. Cell 2000; 6:1287-95.

• Background Reference 04:

  Bochar DA, Wang L, Beniya H, et al. BRCA1 is associated with a human SWI/SNF-related complex: linking chromatin remodeling to breast cancer. Cell 2000; 102:257-65.

• Other Product Names:

  SMARCA4 Antibody: BRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A, BRG1, SNF2B, Transcription activator BRG1, ATP-dependent helicase SMARCA4

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  192807323

• Physical Properties:

  Liquid