STAT1 Antibody

• Background:

  STAT1 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

• NCBI Gene ID:

  6772

• Swiss Prot:

  P42224

• Accession Number:

  NP_009330

• Host:

  Rabbit

• Reactivity:

  Human, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human STAT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer , Inflammasomes

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store STAT1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  87 kDa, 83 kDa

• Applications Notes:

  STAT1 antibody can be used for detection of STAT1 by ELISA at 1:62500. STAT1 antibody can be used for detection of STAT1 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  STAT1

• NCBI Official Name:

  Signal transducer and activator of transcription 1, 91kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Battle, T.E., (2006) Cancer Res. 66 (7), 3649-3657.

• Other Product Names:

  STAT1, DKFZp686B04100, ISGF-3, STAT91, CANDF7

• Tested Applications:

  ELISA, WB

• Protein ID:

  6274552

• Physical Properties:

  Liquid