STAT1 alpha Antibody

• Background:

  STAT1 alpha Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-α , IFN-β , IFN-γ and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1) .

• NCBI Gene ID:

  6772

• Swiss Prot:

  P42224

• Accession Number:

  NP_009330

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  1021

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar STAT1 alpha expression profile in human cell lines detected by two independent anti-STAT1 alpha antibodies that recognize different epitopes, 1021 against C-terminus domain and competitor antibody against C-terminus domain. STAT1 alpha proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1021 competitor antibody

• Field of Research:

  Signal Transduction

• Purification:

  STAT1 alpha Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (94%)

• Buffer:

  STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  STAT1 alpha antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform.

• Isoform:

  Human STAT1 alpha has two isoforms, including isoform 1 (750aa, 87.3kD) and isoform 2 (712aa, 83kD) . Mouse STAT1 alpha has one isoform (749aa, 87.2kD) and Rat STAT1 alpha also has one isoform (749aa, 87.2kD) . 1021 can detect longer human isoform, mouse and rat, but it cannot detect the shorter human isoform.

• Symbol:

  STAT1

• Positive Control 2:

  Cat. No. 1205 - Jurkat Cell Lysate

• Positive Control 3:

  Cat. No. 1202 - A431 Cell Lysate

• Positive Control 4:

  Cat. No. 1204 - K562 Cell Lysate

• Positive Control 5:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  Signal transducer and activator of transcription 1, 91kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Leonard and O'Shea. Annu. Rev. Immunol. 1998; 16:293-322.

• Background Reference 02:

  Schindler and Darnell. Annu. Rev. Biochem. 1995; 64:621-51.

• Background Reference 03:

  Darnell. Science 1997; 277:1630-5.

• Background Reference 04:

  Schindler et al. Proc. Natl. Acad. Sci. USA 1992; 89:7836-9.

• Other Product Names:

  STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91

• Tested Applications:

  ELISA, WB, ICC, IP, IHC-P, IF

• Protein ID:

  6274552

• Physical Properties:

  Liquid