STAT1 (Ab 701) Antibody

• Background:

  Signal transducer and activator of transcription that mediates signaling by interferons (IFNs) . Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-γ -activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

• NCBI Gene ID:

  6772

• Swiss Prot:

  P42224

• Accession Number:

  NP_009330.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  STAT1 (Ab-701) antibody was raised against a peptide sequence around aa. 699~703 (T-G-Y-I-K) derived from Human STAT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer , Inflammasomes

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  91 kDa

• Specificity:

  This antibody detects endogenous level of total STAT1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  STAT1

• NCBI Official Name:

  Signal transducer and activator of transcription 1, 91kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Meraz M A, et al. (1996) Cell. 84: 431-442. Wakao H, et al. (1994) EMBO J. 13: 2182-2191. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.

• Other Product Names:

  CANDF7, ISGF-3, STAT91, TRKB, Transcription factor ISGF-3 components p91/p84

• Tested Applications:

  WB, IHC

• Protein ID:

  6274552

• Physical Properties:

  Liquid