PSMC3 Antibody

• Background:

  PSMC3 is a subunit of the 26S proteasome. 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9

• NCBI Gene ID:

  5702

• Swiss Prot:

  Q6IBS1

• Accession Number:

  NP_002795

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSMC3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PSMC3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  45 kDa

• Applications Notes:

  PSMC3 antibody can be used for detection of PSMC3 by ELISA at 1:312500. PSMC3 antibody can be used for detection of PSMC3 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PSMC3

• NCBI Official Name:

  Proteasome (prosome, macropain) 26S subunit, ATPase, 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Thompson, H.G., et al., (2004) Int. J. Cancer 111 (3), 338-347.

• Other Product Names:

  PSMC3, TBP1

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  21361144

• Physical Properties:

  Liquid