NT5C3 Antibody

• Background:

  Pyrimidine 5-prime-nucleotidase (P5N), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2) . The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology. Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2; MIM 191720) . The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.

• NCBI Gene ID:

  51251

• Swiss Prot:

  Q9H0P0-1

• Accession Number:

  NP_001002009

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NT5C3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NT5C3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  34 kDa

• Applications Notes:

  NT5C3 antibody can be used for detection of NT5C3 by ELISA at 1:1562500. NT5C3 antibody can be used for detection of NT5C3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NT5C3A

• NCBI Official Name:

  5'-nucleotidase, cytosolic IIIA

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chondrogianni, N. (2007) Exp. Gerontol. 42 (9), 899-903.

• Other Product Names:

  NT5C3, MGC27337, MGC87109, MGC87828, P5'N-1, PN-I, PSN1, UMPH, UMPH1, cN-III, p36, POMP, NT5C3, P5N-1, hUMP1

• Tested Applications:

  ELISA, WB

• Protein ID:

  70608080

• Physical Properties:

  Liquid