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SMAD5 Antibody

CAT: 0223-27-476Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-27-476Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
NCBI Gene ID
4090
Swiss Prot
Q99717
Accession Number
NP_001001420
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer, Signal Transduction
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SMAD5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
51 kDa, 52 kDa, 52 kDa
Applications Notes
SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
SMAD5
NCBI Official Name
SMAD family member 5
NCBI Organism
Homo sapiens
Background Reference 01
Langenfeld, E.M., et al., (2006) Oncogene 25 (5), 685-692.
Other Product Names
SMAD5, DWFC, JV5-1, MADH5
Tested Applications
ELISA, WB
Protein ID
47778931
Physical Properties
Liquid

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