NARF Antibody

• Background:

  Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

• NCBI Gene ID:

  26502

• Swiss Prot:

  Q9UHQ1-2

• Accession Number:

  NP_114174

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NARF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. XBL-10422 - Fetal Spleen Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NARF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  55 kDa

• Applications Notes:

  NARF antibody can be used for detection of NARF by ELISA at 1:312500. NARF antibody can be used for detection of NARF by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NARF

• NCBI Official Name:

  Nuclear prelamin A recognition factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lattanzi, G., (2007) J. Cell. Biochem. 102 (5), 1149-1159.

• Other Product Names:

  NARF, DKFZp434G0420, FLJ10067, IOP2

• Tested Applications:

  ELISA, WB

• Protein ID:

  14165461

• Physical Properties:

  Liquid