NARF Antibody

• Background:

  Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

• NCBI Gene ID:

  26502

• Swiss Prot:

  Q9UHQ1

• Accession Number:

  Q9UHQ1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human NARF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  51 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NARF

• NCBI Official Name:

  Nuclear prelamin A recognition factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02:

  Moller-Krull, M., et al. J. Mol. Biol. 382 (3) :601-609 (2008)

• Background Reference 03:

  Melzer, D., et al. PLoS Genet. (5), E1000072 (2008) :

• Background Reference 04:

  Lattanzi, G., et al. J. Cell. Biochem. 102 (5) :1149-1159 (2007)

• Other Product Names:

  Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2, IOP2, NARF

• Tested Applications:

  WB

• Protein ID:

  74735021

• Physical Properties:

  Liquid