ALG1 Antibody

• Background:

  ALG1 catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K) .The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. Mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.

• NCBI Gene ID:

  56052

• Swiss Prot:

  Q9BT22

• Accession Number:

  NP_061982

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALG1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  NTERA2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ALG1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  52 kDa

• Applications Notes:

  ALG1 antibody can be used for detection of ALG1 by ELISA at 1:62500. ALG1 antibody can be used for detection of ALG1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ALG1

• NCBI Official Name:

  ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Grubenmann, C.E., (2004) Hum. Mol. Genet. 13 (5), 535-542.

• Other Product Names:

  ALG1, HMAT1, HMT-1, HMT1, MT-1, CDG1K, Mat-1, hMat-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  41350216

• Physical Properties:

  Liquid