NDE1 Antibody

• Background:

  NDE1 is required for centrosome duplication and formation and function of the mitotic spindle. It is essential for the development of the cerebral cortex. NDE1 may regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex

• NCBI Gene ID:

  54820

• Swiss Prot:

  Q9NXR1-2

• Accession Number:

  NP_060138

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NDE1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NDE1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  38 kDa

• Applications Notes:

  NDE1 antibody can be used for detection of NDE1 by ELISA at 1:62500. NDE1 antibody can be used for detection of NDE1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NDE1

• NCBI Official Name:

  NudE neurodevelopment protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Burdick, K.E., (er) Hum. Mol. Genet. (2008) In press.

• Other Product Names:

  NDE1, FLJ20101, HOM-TES-87, NUDE, NUDE1, NDE, LIS4

• Tested Applications:

  ELISA, WB

• Protein ID:

  8923110

• Physical Properties:

  Liquid