DPF3 Antibody

• Background:

  DPF3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) . DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac) . In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.

• NCBI Gene ID:

  8110

• Swiss Prot:

  Q92784

• Accession Number:

  NP_036206

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPF3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1219 - MCF7 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DPF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  40 kDa

• Applications Notes:

  DPF3 antibody can be used for detection of DPF3 by ELISA at 1:12500. DPF3 antibody can be used for detection of DPF3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  DPF3

• NCBI Official Name:

  D4, zinc and double PHD fingers, family 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hoyal, C.R., (er) J Carcinog 4, 13 (2005) .

• Other Product Names:

  DPF3, CERD4, FLJ14079, BAF45C

• Tested Applications:

  ELISA, WB

• Protein ID:

  148762958

• Physical Properties:

  Liquid