TSFM Antibody

• Background:

  The TSFM protein is a mitochondrial translation elongation factor. Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes.Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]) .

• NCBI Gene ID:

  10102

• Swiss Prot:

  P43897

• Accession Number:

  NP_005717

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TSFM.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TSFM antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  38 kDa

• Applications Notes:

  TSFM antibody can be used for detection of TSFM by ELISA at 1:312500. TSFM antibody can be used for detection of TSFM by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TSFM

• NCBI Official Name:

  Ts translation elongation factor, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Smeitink, J.A., (2006) Am. J. Hum. Genet. 79 (5), 869-877.

• Other Product Names:

  TSFM, COXPD3, EF-TS, EF-Tsmt, EFTS, EFTSMT

• Tested Applications:

  ELISA, WB

• Protein ID:

  171846268

• Physical Properties:

  Liquid