PYGL Antibody

• Background:

  This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

• NCBI Gene ID:

  5836

• Swiss Prot:

  P06737

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PYGL (NP_002854.3) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  HL-60

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 110kDa

• Fragment:

  IgG

• Symbol:

  PYGL

• Positive Control 2:

  SKOV3

• Positive Control 3:

  HepG2

• Positive Control 4:

  Mouse liver

• Positive Control 5:

  Mouse lung

• Positive Control 6:

  Rat liver

• NCBI Official Name:

  Phosphorylase, glycogen, liver

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  PYGL, GPLL, Phosphorylase, glycogen liver, Phosphorylase, glycogen, liver, GSD6

• Tested Applications:

  WB, IF

• Physical Properties:

  Liquid