RAG2 Antibody

• Background:

  This gene encodes a protein that is involved in the initiation of V (D) J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

• NCBI Gene ID:

  5897

• Swiss Prot:

  P55895

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_001230715.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity purification

• Positive Control:

  Raji

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 59kDa

• Fragment:

  IgG

• Symbol:

  RAG2

• Positive Control 2:

  Jurkat

• Positive Control 3:

  293T

• Positive Control 4:

  HepG2

• NCBI Official Name:

  Recombination activating 2

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  RAG-2, V (D) J recombination-activating protein 2, recombination activating gene 2

• Tested Applications:

  WB, IF

• Physical Properties:

  Liquid