AKT1 Antibody, KO Validated

• Background:

  The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

• NCBI Gene ID:

  207

• Swiss Prot:

  P31749

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide of human AKT1

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Antibody is Knockout validated.

• Field of Research:

  Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  Mouse thymus

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 56kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

• Symbol:

  AKT1

• Positive Control 2:

  Rat thymus

• NCBI Official Name:

  V-akt murine thymoma viral oncogene homolog 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Akt1 Antibody: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, Protein kinase B

• Tested Applications:

  WB, IHC

• Physical Properties:

  Liquid