GCAT Antibody

• Background:

  The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.

• NCBI Gene ID:

  23464

• Swiss Prot:

  O75600

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 170-419 of human GCAT (NP_055106.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  Mouse eye

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 45kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  GCAT

• NCBI Official Name:

  2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL

• Tested Applications:

  WB

• Physical Properties:

  Liquid