HLA-E Antibody

• Background:

  HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

• NCBI Gene ID:

  3133

• Swiss Prot:

  P13747

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 22-200 of human HLA-E (NP_005507.3) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  Affinity purification

• Positive Control:

  HL-60

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 50kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  HLA-E

• Positive Control 2:

  SKOV3

• Positive Control 3:

  Mouse liver

• Positive Control 4:

  Rat liver

• NCBI Official Name:

  Major histocompatibility complex, class I, E

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  HLA-E, EA2.1, HLAE, MHC HLA-E alpha-2.1, MHC HLA-E alpha-1, EA1.2, HLA-6.2, MHC class I antigen E, QA1

• Tested Applications:

  WB

• Physical Properties:

  Liquid