POLR2A Antibody

• Background:

  This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

• NCBI Gene ID:

  5430

• Swiss Prot:

  P24928

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Pig

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Affinity purification

• Positive Control:

  HeLa

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 250kDa

• Fragment:

  IgG

• Symbol:

  POLR2A

• Positive Control 2:

  BT-474

• Positive Control 3:

  H460

• Positive Control 4:

  Jurkat

• Positive Control 5:

  Mouse testis

• Positive Control 6:

  Mouse brain

• NCBI Official Name:

  Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

• Tested Applications:

  WB, IHC, IF, IP, ChIP

• Physical Properties:

  Liquid